Several molecular-biological techniques are employed to
characterize transgene expression in transgenic plants and
animals. However, no technique is available to detect expression
of a transgene product in a tissue-specific manner.
Histochemistry is a powerful technique for localization of
trace quantities of substances present in biological tissues
(Pearse 1972, 1988; Krishnamurthy 1998). Histochemical
techniques have been employed to characterize structure and
development, and to study time course of deposition and
distribution of major storage compounds such as protein,
lipid, starch, phytin, and minerals such as calcium, potassium
and iron in rice grains (Krishnan et al. 2001; Krishnan
and Dayanandan 2003).