Molecular Phylogeny of mangroves III Parentage analysis of a Rhizophora hybrid using random amplified polymorphic DNA and restriction fragment length polymorphism markers

TitleMolecular Phylogeny of mangroves III Parentage analysis of a Rhizophora hybrid using random amplified polymorphic DNA and restriction fragment length polymorphism markers
Publication TypeJournal Article
Year of Publication1997
AuthorsParani M, Rao CSrinivasa, Mathan N, Anuratha CS, Narayanan KK, Parida A
Journal. Aquatic Botany
Start Page165
End page172
Date Published1997
KeywordsMangroves; Molecular markers: Conservation: Mitochondrial genome
Abstract

In this study, random amplified polymorphic DNA (RAPD) markers of genomic DNA and
restriction fragment length polymorphism (RFLP) of mitochondrial DNA using a mitochondrial
genome specific probe, atp 6 (from maize), were used to identify the parentage of a Rhi=ophora
hybrid. The combined RAPD profiles of two species, Rhi=ophora apicuh~ta and R. mucronata
had 96.5%, similarity with that of the hybrid, indicating that these two species are the probable
parents. RFLPs for the mitochondrial genome were readily observed, and the RFLP profile of R.
apiculata was exactly the same as that of the hybrid, establishing its maternal status for the
hybrid. This is the first report on characterising the parentage of a Rhi=ophora hvbrid using
molecular markers. © 1997 Elsevier Science B.V.
Publisher Category: 
MSSRF Staff
Department: 
Bio-Technology