Random amplified polymorphic DNA (RAPD) markers for genetic analysis in micropropagated plants of Populus deltoides Marsh

TitleRandom amplified polymorphic DNA (RAPD) markers for genetic analysis in micropropagated plants of Populus deltoides Marsh
Publication TypeJournal Article
Year of Publication1995
AuthorsRani V, Parida A, Raina S.N.
JournalPl. Cell Reports
Start Page459
End page462
Date Published1995
KeywordsDNA, genetic fidelity, micropropagated plants, Pl. Cell Reports, Populus deltoides Marsh, Random amplified polymorphic DNA
Abstract

RAPD markers were used to assess genetic fidelity of 23 micropropagated plants of a single clone (L34) of Populus deltoides. Eleven arbitrary 10-base primers were successfully used to amplify DNA from in vivo and in vitro material. Of these, 5 distinguished a total of 13 polymorphisms common across 6 micropropagated plants. Apart from these 6 plants, the amplification products were monomorphic across all the micropropagated plants, the mother plant and 4 additional fieldgrown control plants. Our results show that RAPD markers can be used to gain rapid and precise
information about genetic similarities or dissimilarities in micropropagation systems that might not be so easily evident from other commonly used techniques.

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