Ploidy-depdenten genomic stability in the tissue cultures of ornamental Phlox drummondii Hook

TitlePloidy-depdenten genomic stability in the tissue cultures of ornamental Phlox drummondii Hook
Publication TypeJournal Article
Year of Publication1992
AuthorsRaja VG, KOUL K.K., Raina S.N., Parida A
JournalPl. Cell Reports
Start Page12
End page17
Date Published1992
Keywordsgenomic stability, karyomorphology, Pl. Cell Reports, Ploidy-depdenten, regenerated roots
Abstract

Comparisons of the chromosome numbers, 2C nuclear DNA amounts and karyomorphology were made in explant cultures of diploid (2n = 2,x = 14) and autotetraploid (2n = 4x = 28) Phlox drummondii. In 6-36 week old calli derived from diploid intemodai segment explants, and in cells of root tips regenerated from such callus, marked differences were observed in chromosome number. The chromosome numbers ranged from 2n = 14 to 2n = 100 and DNA amounts from 8.20 to 63.20 pg in the diploid derived callus, while the extent of variation was much reduced in the regenerated roots. In contrast, the autotetraploid cultures were characterised by the maintenance of the same chromosome number and DNA amounts as the mother plant. Modified chromosome structures were not apparent in any of the cultures. The possible reasons for the chromosomal instability at the diploid level and stability at the tetraploid level are discussed.

http://www.ncbi.nlm.nih.gov/pubmed/24201722

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