Random amplified polymorphic DNA (RAPD) markers for genetic analysis in micropropagated plants of Populus deltoides Marsh
Title | Random amplified polymorphic DNA (RAPD) markers for genetic analysis in micropropagated plants of Populus deltoides Marsh |
Publication Type | Journal Article |
Year of Publication | 1995 |
Authors | Vijay R, Ajay P, Raina S.N. |
Journal | Plant Cell Reports |
Volume | 14 |
Start Page | 459 |
Issue | 7 |
End page | 462 |
Date Published | 04/1995 |
Keywords | DNA (RAPD), genetic analysis, micropropagated plants, Plant Cell Reports, Populus deltoides Marsh, Random amplified |
Abstract | RAPD markers were used to assess genetic fidelity of 23 micropropagated plants of a single clone (L34) of Populus deltoides. Eleven arbitrary 10-base primers were successfully used to amplify DNA from in vivo and in vitro material. Of these, 5 distinguished a total of 13 polymorphisms common across 6 micropropagated plants. Apart from these 6 plants, the amplification products were monomorphic across all the micropropagated plants, the mother plant and 4 additional fieldgrown control plants. Our results show that RAPD markers can be used to gain rapid and precise |