The indiscriminate use of pesticides to control major pests and diseases has led to several adverse consequences and deepened agrarian distress, polluted the environment and created a heavy disturbance in the ecosystem, Ultimately the end users (human and animal beings) are also severely affected. Hence, it is essential to remove these chemo-pollutant from the environment. Biological removal of chemo-pollutants becomes the safest and easiest method. In the present study, some of the traditional plants with medicinal properties viz. Cipadessa baccifera, Clausena dentata, Dodonaea angustifolia and Melia dubia along with Trichoderma viride were tested for their capacity to degrade the commonly used pesticides namely, endosulfan, acephate and quinalphos under in vitro conditions. The pH, nutritional status and the microflora (bacteria and fungal population) of the soil were analysed from zero to 60th day at 20 days interval. The residues of these pesticides detected by gas chromatography revealed that the above mentioned plants along with Trichoderma were highly efficient in degrading the pesticides. Improvement in the soil health condition (microbial community) is also proved that the biological method of degradation is safe to the ecosystem.