The study was carried out on antimicrobial, antioxidant and anticancer activity of Syzygium benthamianum leaf extract. Chemical compositions of the leaf were analysed using GC/MS technique. A total of 24 compounds were identified among which 4-(4-ethylcyclohexyl)-1-pentyl-Cyclohexene (24.07 %) and Linoleic acid (15.16 %) are the major constituents. Antimicrobial activity of the leaf was observed on six bacterial and three fungal species, whose MIC values ranged from 100 to 500 μg/ml. At higher concentration, the extract exhibits higher scavenging activity (94.7 %) that was comparable with standard BHT. It was also observed that the leaf sample were able to effectively inhibit the growth of Hep 2 cells.