Rapid comparisons of the chromosome number by genome size determination were made
in the explant source and 1400 enhanced axillary branching-derived plants of Populus
deltoides clones, P. ciliata, Eucalyptus tereticornis, E. camaldulensis, Anogeissus
pendula, Alnus nepalensis, Prosopis cinerarea, Wrightia tomentosa, W. tinctoria and
Tecomella undulata. The estimated DNA amounts in the meristematic zones of root and
shoot tips of each of the plants, collected at various cycles of tissue culture from
multiplication to field transfer, complemented with the DNA amount of the zygotic
chromosome number (2n) of the respective species. RAPD fingerprinting, however,
revealed polymorphism among micropropagated plants of two P. deltoides clones. This
emphasizes the need for genetic analysis of micropropagated plants by more than one
approach before a specific protocol is released for mass multiplication.

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