Jatropha curcas L. is an important non-edible oil yielding plant growing in wasteland and dry lands. The
experiment was conducted to study the plant regeneration of J. curcas from different ages of petioles.
Petioles explant grown on Murashige and Skoog’s (MS) medium supplemented with 1 mg/l α-
naphthalene acetic acid and 0.2 mg/l kinetin showed the highest frequency of callus induction and the
same medium was found to be best suited for callus growth in 1st petiole. Percent of somatic embryo
formation was higher (35.16%) from the 1st petiole explant on 0.5 mg/l thidiazuron with 0.4 mg/l
gibberellic acid. MS medium supplemented with 1 mg/l N6-benzylaminopurine, 0.5 mg/l indole-3-acetic
acid, 0.25 mg/l kinetin recorded the higher somatic embryo germination percentage (66.85%). MS basal
medium supplemented with 0.5 mg/l thidiazuron, 0.1 mg/l N6-benzylaminopurine, 0.4 mg/l gibberellic acid
was found to be best medium for shoot elongation. Among the auxins tested, the higher frequency of
root formation was observed in MS medium supplemented with 0.3 mg/l α-naphthalene acetic acid
compared to indole-3-butyric acid and indole-3-acetic acid. The survival rate of in vitro rooted plantlets
was dependent on the type of explant source and it varies from 47 to 60%.