Among the numerous microorganisms in the soil, earthworms play an important role in the formation and maintenance of soil structure and fertility by altering the physical, chemical and biological properties. A study was conducted in Wayanad district during 2017 to 2018 to collect and identify the earthworm species present in three agro climatic regions of Wayanad. A total of 30 samples were collected from Wetland, upland and an evergreen forest. A total of 15 earth worm species were identified and among this two species were new to Wayanad, the Dravida thomasi and Amynthas corticis.