Neanotis prabhuii, a new species from the Western Ghats of Kerala, India, is described. The species resembles N. wightiana in its compressed, glabrous and indehiscent capsule, but differs in having quadrangular broadly winged stem, large leaf lamina with cordate to amplexicaule base and conspicuous 4–6 pairs of lateral veins, dichasial corymbose cyme, large and heterostylous flowers with bristly hairs on corolla lobes, long staminal filament and style. Notes on distribution, phenology along with comparison of its allied species are also provided.